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However, we recommend that you experiment with the timing to see what works best for you

J Phys Chem 121(26):63596366

For general guidance, the following buffered aqueous conditions may be applicable: 10-250 mM NaCl, 5-50 mM Tris HCl, pH 5-8, with optional addition of divalent cation(s) and/or metal chelators and/or non- ionic detergents and/or membrane fractions and/or anti-foam agents and/or scintillants